Fitc dmso

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August undefined, 2024

WebApr 11, 2024 · 中文名称：荧光素-聚乙二醇-双棕榈酸甘油酯英文名称：fitc-peg-dpg 外观：取决于分子量溶解性：water, dmso, dcm 纯度：95% 活性基团：fitc 分子量：400 600 1000 2000 3400 4000 5000 10000 20000 注意事项取用保持低温干燥，现配现用，避免频繁冻融造成产品失活存储条件 -20 ...

磷脂-聚乙二醇-荧光素 DSPE-PEG2000-FITC

WebDespite the growing number of choices in amine-reactive fluorophores, fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) are still widely used reactive fluorescent dyes for preparing fluorescent bioconjugates. ... (DMSO) and should never be used in that solvent. Figure 1.1.8. Reaction of a primary amine with a ... WebAug 1, 2024 · For DMSO/DBTD method, the fluorescent label is tagged to the hydroxyl group of AX. FITC can be grafted onto AX through thiocarbamoyl linkage, as shown in Fig. 1 a. FITC reacts with AX in DMSO at elevated temperature to give O-(fluoresceinyl-thiocarbamoyl) AX with pyridine and DBTD as the catalysts. During the reaction, pyridine … north carolina renters insurance average cost

FITC (CAS 3326-32-7): R&D Systems

WebDec 11, 2024 · Dissolve FITC in anhydrous DMSO at 1 mg/ml concentration. This should be prepared fresh for each labelling reaction. Add 5 μl of the dye to the dialysed protein (5 … Web1.8 Mg of PU-FITC in an amber Eppendorf tube was dissolved in 200 μL of DMSO [molecular biology grade from Sigma Aldrich] and vortexed to make 10 mM PU-FITC solution. This 10 mM PU-FITC solution was further diluted (10-fold) with DMSO to make 2 mL of 1 mM PU-FITC solution and stored as small aliquots of 50 μL in 40 amber … http://www.qiyuebio.com/product/240649 how to reset carmd

Introduction to Amine Modification—Section 1.1 Thermo …

FITC-PEG(3)-NH2 - 西安瑞禧生物技术有限公司

WebAug 5, 2013 · The FCS study reveals a change of the hydrodynamic radius of BSA from 3.7 nm in the native state to 7.0 nm in the presence of 40% DMSO, which suggests complete unfolding of the protein under these conditions. Fluorescence self-quenching of FITC has been exploited to understand the conformational dynamics of BSA. WebNov 22, 2011 · In vitro disease modeling using pluripotent stem cells can be a fast track screening tool for toxicological testing of candidate drug molecules. Dimethyl sulfoxide (DMSO) is one of the most commonly used solvents in drug screening. In the present investigation, we exposed 14- to 21-day-old embryoid bodies (EBs) to three different … how to reset car keyWebFITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu2+-sensitive fluorescence dye. - Mechanism of Action & Protocol. ... Add each solvent one by one: 10% DMSO 90% … how to reset car in beamng drive

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Fitc dmso

Fluorescein Isothiocyanate - an overview ScienceDirect Topics

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WebFITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines. λ Ex /λ Em = 492/520 nm. ε = 75,000. Orange solid soluble in DMF or DMSO. Store at -20°C and protect from light, especially in solution. WebDMSO: 10 mg/mL (2.5mM), Need ultrasonic ( < 1 mg/ml refers to the product slightly soluble or insoluble ) 参考文献. 1. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. ... (FITC)-Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations. Transl Stroke Res. 2011 Mar;2(1):106-11.

WebFITC: fluorescein isothiocyanate; used as a fluorescent label for proteins, especially antibodies. WebFITC is a fluorescent reagent for the labeling of amines. Excitation maximum ~ 495 nm; emission maximum ~ 525 nm. Optical Data for FITC. ... Soluble to 100 mM in DMSO. Purity: ≥95%. Storage: Store at -20°C. CAS No: …

WebDimethyl sulfoxide, or DMSO, is a highly polar organic solvent that is miscible with water. ... (FITC)) • Thiol-reactive fluorescent labeling reagents (e.g. Alexa Fluor™ 488 or fluorescein maleimide) Molecular Probes™ … WebDec 12, 2024 · Dissolve FITC in anhydrous DMSO at 1 mg/ml concentration. This should be prepared fresh for each labelling reaction. Add 5 μl of the dye to the dialysed protein (5 ml) every 30 min to get a final concentration of 100 ng FITC/1 μg protein. Perform this step by gently rocking the protein solution at 4°C for 12 h.

Web西安齐岳生物科技有限公司生产销售“磷脂-聚乙二醇-荧光素 dspe-peg2000-fitc”-“产品名称磷脂-聚乙二醇-荧光素 dspe-peg-fitc 中文名称荧光素聚乙二醇磷脂磷脂peg荧光素英文名称 fitc-peg-dspe dspe-peg-fitc 溶解度溶于dmso dmf dcm等有机溶剂”，该产品仅用于科研，如果需要请联系我们

WebFITC is covalently coupled to primary amines (lysines) of the immunoglobulin. Dissolve 10 mgs (the entire contents of 1 vial; no need to weigh) of FITC in 1 mL anhydrous DMSO … north carolina rep. ted buddWebProtocol of conjugation of FITC to peptide amino groups in solution. Reagents: DIPEA/TEA, DMF/DMSO, FITC, peptide. 1. Dissolve peptide (approx. 1 mg/ml; 2 mM or less) and FITC (1.5-3 eq.) in DMF or DMSO. 2. Add TEA or DIPEA (25 eq.) and keep in dark for at least 4 hours. Reaction time can be extended. Control reaction progress using e.g. TLC. 3. north carolina republican senate debateWeb西安齐岳生物科技有限公司生产销售“四臂聚乙二醇荧光素 4ARM-PEG-FITC”-“中文名称四臂聚乙二醇荧光素四臂PEG荧光素英文名称 4ARM-PEG-FITC 4Arm-PEG-Fluoresein 溶解度溶于DMSO DMF DCM等有机溶剂，溶于水”，该产品仅用于科研，如果需要请联系我们 . north carolina republican primary resultsWebFITC is soluble in DMSO and DMF. Applications. General. FITC is commonly used for labeling antibodies 2 (IgG) and also in other immunological applications. It is also often used in flow cytometry, a technology frequently used by biologists to study cellular populations with high precision 3. north carolina replacement medicaid cardhttp://www.qiyuebio.com/product/240649 how to reset car maintenance lightWebDMSO: 5 mg/mL DMF: soluble aqueous buffer, pH ≥ 9.0: soluble. storage temp. 2-8°C. ... various proteins, and oligopeptides with fluorescein. FITC-antibody conjugates are useful in many applications, including flow cytometry and immunohistochemistry (excitation maximum = 492 nm; emission maximum = 520 nm). λ max: 492 ± 3 nm (pH 9 ... north carolina rentals beachWebApr 11, 2024 · 荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔(Delta)生物试剂定制生产，产品长期供货。荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔(Delta)生物试剂仅用于科研。荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔(Delta)生物试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购荧光素聚乙二醇巯基 FITC-PEG-SH-德尔塔 ... how to reset casio fx-350es plus