WebMar 1, 1992 · Halolysin has a long C-terminal extension of approximately 120 amino acids which has not been found in other extracellular subtilisin type serine proteases. The gene, hly, was expressed in another ... WebMay 23, 1996 · A gene encoding a halophilic serine proteinase, halolysin R4, from a halophilic archaeon Haloferax mediterranei strain R4 was cloned, its nucleotide …
Functional Insight into the C-Terminal Extension of Halolysin …
Webjournals.asm.org WebYet the diversity of halolysins remains underexplored. In this study, a halolysin from the extremely halophilic archaeon Haladaptatus sp. DYF46 (Hly Hap) was identified to be a … marks mobility centre
Biochemical characterization of a low salt-adapted extracellular ...
WebAug 3, 2024 · Halolysin SptA, the major extracellular protease of Natrinema sp. J7-2, is capable of intracellular self-activation to affect haloarchaeal growth. Here, we report that … Halolysin precursors contain a twin arginine signal peptide, a propeptide, a subtilase catalytic domain and a C-terminal extension (CTE). During or after the trans-membrane transport, they are autocatalytically cleaved to generate a mature halolysin. The mature halolysin is composed of a catalytic … See more BlastP analysis with the classical halolysin Nep from Natrialba magadii as a query showed that five potential genes encoding halolysins without CTE were present … See more To investigate the effect of CTE on HlyHap activity, HlyHap was heterologously expressed with the CTE of HlyA from Halococcus salifodinae (123 aa) (Hou et al. … See more HlyHap precursor was expressed in E. coli and purified by affinity chromatography. The purified HlyHap precursor did not possess protease activity. SDS … See more The optimal NaCl concentration for HlyHap was 0.25-0.50 M (Fig. 4a). The enzyme activity decreased with the increase of NaCl concentration. When NaCl … See more WebFeb 12, 2024 · Importance: This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, which expands our knowledge on the traditional function of haloarchaeal extracellular proteases. Haloarchaeal extracellular serine proteases have been previously discussed as growth … marks mobile jefferson city mo