WebbPush-button Spring Panel mounted 32,5 N 0,37 VA13-HIS4 Red Push-button Spring Panel mounted 32,5 N 0,37 VA13-HIS4A06* Black Push-button Air signal Panel mounted 6 … WebbABSTRACT. The HIS4 gene in Saccharomyces cerevisiaewas put under the transcriptional control of RNA polymerase I to determine the in vivo consequences on mRNA processing and gene expression.This gene, referred to as rhis4, was substituted for the normal HIS4 gene on chromosome III. Therhis4 gene transcribes two mRNAs, of which each initiates …
毕赤酵母同源重组的原理及目的基因整合方式 - 豆丁网
Webb4 maj 2024 · EC Number. 1.1.1.23, 3.5.4.19, 3.6.1.31. Experimental Data. Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. WebbHis 标签 (His-tag) 又称多组氨酸标签，由 6 到 10 个连续的组氨酸残基组成。最常用的 his 标签是 6xHis 标签，其分子量为 0.8 kDa 1 。 该标签用于许多重组蛋白，可帮助蛋白纯 … maverick mcwilliams
RNA Polymerase I-Promoted HIS4 Expression Yields Uncapped ...
WebbHIS4 selection in Pichia For insertion at HIS4, linearize with Sal I or Stu I (generates His+ Mut+ in GS115 and His+ MutS in KM71) For a gene replacement at AOX1 in GS115, linearize with Bgl II (generates His+ MutS) See page 24 for alternate restriction sites if your insert DNA has a Bgl II, Stu I, or WebbHIS4, LEU2, ARG4, TRP1, and . URA3. all complement their respective mutant genes in . Pichia. Pichia pastoris as a methylotrophic yeast . Pichia pastoris is a methylotrophic. … Webb24 maj 2009 · It has a mutation in the histinol dehydrogenase gene (HIS4) and was generated by nitrosoguanidine mutagenesis at Phillips Petroleum Co 35. It is the most frequently used Pichia strain for ... herman miller workshop